Fig. 2.
Phospho-p38 is expressed by microglia in the spinal cord after plantar incision. High-power images from the ipsilateral spinal dorsal horn 3 h after adult incision (IN) (Ai–Aiii) and neonatal plus adult incision (nIN-IN) (Biv–Bvii). Double labeling demonstrates colocalization of p-p38 with the microglial marker ionized calcium-binding adaptor molecule (Iba1) (i, iv), confirmed in xz, xy, and yz planes (vii). Phospho-p38 is not coexpressed with glial (glial fibrillary acid protein [GFAP]) or neuronal (NeuN) markers at this time point. Scale bar = 50 μm.