Fig. 7.
Effects of protease-activated receptors-1 (PAR1) antagonist on thrombin- or TFLLR-NH2-induced proenkephalin expression in HaCaT cells. Cells were incubated with thrombin (5 U/ml), PAR1 antagonist SCH79797 (5 μM), PAR1 agonist TFLLR-NH2 (100 μM), at 37°C for a period of 6 h. Proenkephalin messenger RNA (mRNA) (A) and protein (B) expressions were evaluated by real-time reverse-transcription polymerase chain reaction (n = 5 in each group) and immunoblotting analysis (n = 4 in each group). Results indicate that PAR1 agonist and thrombin increased proenkephalin expression in HaCaT cells, whereas SCH79797 inhibited thrombin-induced proenkephalin mRNA and protein expression. Data are expressed as mean ± SEM. Ctr = control; Sch = SCH79797; TF = TFLLR-NH2; Th = thrombin.