Fig. 6.
Genetic deletion of Hsp72 attenuates the effect of the heat-shock response on lung vascular permeability and bacterial clearance in a mouse model of Pseudomonas aeruginosa pneumonia. (A–D) C57BL/6 wild-type (WT) or Hsp72-null mice were left untreated or underwent a heat-shock response with whole-body hyperthermia that was followed 24 h later by airspace instillation of P. aeruginosa (PAK strain, 1 × 107 colony-forming units [CFU]) or its vehicle. Mice were euthanized 4 h later and excess lung water (ELW) and lung vascular protein permeability (EVPE) were measured, as described in the Materials and Methods section. In separate experiments, bronchial alveolar lavage (BAL) was performed and cells counted and BAL fluid protein concentration measured. For all experiments, results are shown as mean ± SD (n = 6 mice per group); *P ≤ 0.05 from controls, **P≤ 0.05 from mice with PAK alone.