Fig. 5.
Activation of the heat-shock response inhibits phagocytosis of Pseudomonas aeruginosa by mouse neutrophils. (A) Primary mouse neutrophils were isolated, heat-shocked at 42°C for 30 min, and then exposed 60 min later to P. aeruginosa expressing green fluorescent protein (PAK-GFP) for 30 min. After cell fixation, permeabilization, and staining with phalloidin, the number of intracellular PAK-GFP was counted, as described in the Materials and Methods section. Results are shown as mean ± SD (n = 3 experiments repeated in triplicate); *P ≤ 0.05 from non–heat-shocked cells. (B) This provides an example of the inhibition of PAK-GFP bacteria phagocytosis by primary mouse neutrophils that underwent prior heat shock activation. Please note that although PAK-GFP bacteria are stuck to the outside of heat-shocked neutrophils, they have not been endocytosed.

Activation of the heat-shock response inhibits phagocytosis of Pseudomonas aeruginosa by mouse neutrophils. (A) Primary mouse neutrophils were isolated, heat-shocked at 42°C for 30 min, and then exposed 60 min later to P. aeruginosa expressing green fluorescent protein (PAK-GFP) for 30 min. After cell fixation, permeabilization, and staining with phalloidin, the number of intracellular PAK-GFP was counted, as described in the Materials and Methods section. Results are shown as mean ± SD (n = 3 experiments repeated in triplicate); *P ≤ 0.05 from non–heat-shocked cells. (B) This provides an example of the inhibition of PAK-GFP bacteria phagocytosis by primary mouse neutrophils that underwent prior heat shock activation. Please note that although PAK-GFP bacteria are stuck to the outside of heat-shocked neutrophils, they have not been endocytosed.

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