Influence of intraperitoneal leptin treatment (1 μg/g body weight [BW], twice a day) on cellular immune response in the lung after cecal ligation and puncture (CLP)–induced sepsis. Bronchoalveolar lavage fluid from leptin-treated and vehicle (sodium chloride 0.9%)-injected control mice was collected 6 h (n = 5 per group) and 24 h (n = 4 per group) after CLP. Numbers of (A) leukocytes, (B) neutrophils, (C) monocytes, (D) macrophages, (E) CD4 T cells, (F) CD8-T cells, (G) γδ-T cells, and (H) natural killer (NK) cells were enumerated by flow cytometric analysis. All values are expressed as mean ± SEM; *P < 0.05 of two-way ANOVA for unmatched samples followed by a post hoc Bonferroni test for analyses between more than two groups or within groups over time. nd = not detectable.