American Society of Anesthesiologists

Fig. 1.

$Study design. (A) First experiment: mice were divided in five groups treated with intraperitoneal injection of control liposome versus clodrolip 1 h before high-mobility group box 1 protein (HMGB1) Ag versus saline injection. Control animals received saline injections. The training session of the memory test was performed 30 min after the clodrolip/control liposome injection and 30 min before HMGB1 Ag/saline injection; and the context session was performed 72 h later. (B) Second experiment: mice were divided in four groups treated with anti-HMGB1 versus saline 1 h before tibia fracture. The training session of the memory test was performed 30 min after the intraperitoneal injection and 30 min before tibia fracture. (C) Third experiment: mice were divided in four groups treated with anti-HMGB1 versus saline 1 h before tibia fracture and sacrificed 1 and 24 h after the tibia fracture. Anti-HMGB1 = neutralizing HMGB1 monoclonal antibody; CT-lip = control liposome; HMGB1 Ag = HMGB1 antigen.$

Study design. (A) First experiment: mice were divided in five groups treated with intraperitoneal injection of control liposome versus clodrolip 1 h before high-mobility group box 1 protein (HMGB1) Ag versus saline injection. Control animals received saline injections. The training session of the memory test was performed 30 min after the clodrolip/control liposome injection and 30 min before HMGB1 Ag/saline injection; and the context session was performed 72 h later. (B) Second experiment: mice were divided in four groups treated with anti-HMGB1 versus saline 1 h before tibia fracture. The training session of the memory test was performed 30 min after the intraperitoneal injection and 30 min before tibia fracture. (C) Third experiment: mice were divided in four groups treated with anti-HMGB1 versus saline 1 h before tibia fracture and sacrificed 1 and 24 h after the tibia fracture. Anti-HMGB1 = neutralizing HMGB1 monoclonal antibody; CT-lip = control liposome; HMGB1 Ag = HMGB1 antigen.

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