Fig. 2.
Protein expression in brain cortex 3 h after sham operation or cardiac arrest and cardiopulmonary resuscitation (CA/CPR). Sham = mice that were subjected to sham operation without CA/CPR. NT = mice that were maintained normothermia for 3 h after CA/CPR. TH = mice that were treated with therapeutic hypothermia (TH) for 3 h after CA/CPR. (A) Relative phosphorylated nitric oxide synthase 3 (NOS3) at Ser1177 levels were quantified by dividing the phosphorylated at NOS3 at Ser1177 immunoreactivity by NOS3 immunoreactivity and normalized to values of sham-operated mice. (B) Relative phosphorylated NOS3 at Thr495 levels were quantified by dividing the phosphorylated NOS3 at Thr495 immunoreactivity by NOS3 immunoreactivity and normalized to values of sham-operated mice. (C) Relative phosphorylated vasodilator-stimulated phosphoprotein (VASP) at Ser239 levels were quantified by dividing the phosphorylated VASP at Ser239 immunoreactivity by VASP immunoreactivity and normalized to values of sham-operated mice. (D) Relative phosphorylated Akt at Ser473 levels were quantified by dividing the phosphorylated Akt at Ser473 immunoreactivity by Akt immunoreactivity and normalized to values of sham-operated mice.(E) Relative NOS1 levels were quantified by dividing the NOS1 immunoreactivity by β-tubulin immunoreactivity and normalized to values of sham-operated mice. (F) Relative NOS2 levels were quantified by dividing the NOS2 immunoreactivity by β-tubulin immunoreactivity and normalized to values of sham-operated mice. (G) Relative phosphorylated Akt at Thr308 levels were quantified by dividing the phosphorylated Akt at Thr308 immunoreactivity by Akt immunoreactivity and normalized to values of sham-operated mice. (H) Relative phosphorylated AMP-activated protein kinase α (AMPKα) at Thr172 levels were quantified by dividing the phosphorylated AMPKα at Thr172 immunoreactivity by AMPKα immunoreactivity and normalized to values of sham-operated mice. (I) Relative heat shock protein 90 levels were quantified by dividing the heat shock protein 90 immunoreactivity by β-tubulin immunoreactivity and normalized to values of sham-operated mice. Representative blots are shown of six blots in each group. N = 6–7. *P < 0.05, **P < 0.01, and ***P < 0.001. Data were analyzed using one-way ANOVA with a Bonferroni post hoc test.