Localization of GM1 ganglioside (GM1) in detergent-resistant membranes (DRMs) isolated from propofol- or thiopental-treated neurons and mouse brains. (A) Western blots of DRM (fraction 5) and non-DRM fractions (fraction 11) isolated from neurons (21 days in vitro) incubated for 24h in the presence or absence of 20 µm propofol and 100 µm thiopental after pretreatment with 3 µm bicuculline (Bic) or without bicuculline pretreatment and probed with horseradish peroxidase-conjugated cholera toxin B subunit (CTX–HRP). (B) Propofol (120mg/kg) and thiopental (50mg/kg) were intraperitoneally administered to male C57/B6 mice (6 months old) for 24h. The blot of DRM isolated from synaptosomes prepared from propofol- or thiopental-treated mouse brain, which was proved with CTX–HRP, is shown.