Fig. 6.
Spinal reflex responses to hindpaw stimuli in young adulthood are not altered by prior intrathecal levobupivacaine. (A), Mechanical withdrawal threshold at postnatal day (P)35 following intrathecal (IT) injection on postnatal day 3 (P3 IT) or 7 (P7 IT) of saline or 0.5% levobupivacaine (l-bupi). Data points = box and whiskers (5–95 percentile); n = 6–8 all groups. Values did not differ significantly across treatment groups (P = 0.10 one-way ANOVA). (B) Thermal withdrawal latency at P35 following IT saline or levobupivacaine at P3 or P7. Data points = box and whiskers (5–95 percentile); n = 6–8 all groups. (P = 0.051 one-way ANOVA). (C) (i): Example of electromyography (EMG) recordings in biceps femoris at P35 in response to increasing mechanical stimuli (von Frey hair, vFh 17–20), and (ii) plot of stimulus–response relationship for quantification of area under the curve (AUC) to threshold (vFh 18) and suprathreshold (vFh 19 and 20) stimuli. (D) The quantified reflex response (AUC EMG) did not differ between animals treated with saline or levobupivacaine at P3 or P7 (P = 0.87 one-way ANOVA). Data points = mean ± SEM; n = 6 per group.