Fig. 4. Influence of protein synthesis inhibition (cycloheximide 35 µM) and tissue factor blockade ASIS (50 µg/ml) on LPS (225 µg/ml)–induced shortening of clotting time. The lipopolysaccharide effect was abolished by protein synthesis inhibition or tissue factor blockade. In the absence of lipopolysaccharide, cycloheximide and active site–inhibited factor seven had no effect on clotting time. Data from six volunteers are the means ± SEM. Statistical significance of the lipopolysaccharide-evoked shortening of clotting time is indicated by P values. Controls (CON). ASIS = active site–inhibited factor seven; LPS = lipopolysaccharide.