Fig. 5. CD11c+ alveolar macrophages are required for the full pathophysiologic response to ventilated lung ischemia-reperfusion (I-R) injury. Alveolar macrophages (CD11c+) were depleted by treating transgenic CD11c-DTR mice with diphtheria toxin (DTx) (intraperitoneally at day −1) and these mice (or control mice that received PBS carrier) underwent I-R surgery, and the lungs were assessed for neutrophil infiltration by histopathology 3 h after reperfusion. Each image is representative of histologic images from three independent surgeries. PBS = phosphate buffered saline.