Fig. 2. Spinal nerve ligation (SNL) altered glutamic acid decarboxylase 67 (GAD67) immunoreactivity in the locus coeruleus (LC) and spinal dorsal horn ipsilateral to surgery. Photomicrographs of immunostaining for tyrosine hydroxylase (TH: A  and D ), GAD67 (B  and E ), and their merger (C  and F ) in the LC of normal (A , B , and C ) and SNL (D , E , and F ) rats at 2 weeks after surgery. Photomicrographs of immunostaining for GAD67 in the lumbar spinal dorsal horn of normal (G ) and SNL (H ) rats. Scale bar = 100 μm.