Fig. 2. Characterization of MOR1 overexpressing human H358 non-small cell lung cancer cells. (A ) Control (nontransfected, C), stable vector control (VC) and μ-opioid receptor 1 (MOR1, the most abundant MOR transcript that consists of exons 1, 2, 3, and 434) overexpressing (O/E) H358 cell lines were generated, cell lysates obtained and immunoblotted with anti-μ-opioid receptor (a), anti-epidermal growth factor receptor (b), anti-pTyr1173epidermal growth factor receptor (c), and anti-actin (2-day) antibodies. (B ) Phase-contrast images of H358 control (nontransfected), stable VC, or stable MOR1 O/E cells. The arrows indicate elongated cellular projections suggestive of a migratory phenotype.