Fig. 4. Western blotting and densitometry results. Western blot analysis showing concentrations of total BAD, phosphorylated BAD (Ser 112) (A ), caspase 3 (B ), Bcl-2 (C ), and Pim-1 kinase (D ) after 15 min of reoxygenation alone (control 15-min reoxygenation, n = 6) or in the presence of 50 μM pravastatin (Prava 15-min reoxygenation, n = 6) and after 60 min of reoxygenation alone (control 60-min reoxygenation, n = 6) or in the presence of pravastatin 50 μM (Prava 60-min reoxygenation, n = 6). Equal loading was confirmed by Western blot with an antiglyceraldehyde 3-phosphate dehydrogenase antibody. The relative total BAD, phospho-BAD, caspase 3, Bcl-2, and Pim-1 kinase protein concentrations were calculated by averaging the results obtained from six independent experiments for each time. Data are presented as the mean ± SD. *P < 0.01 versus  control 15- min reoxygenation group. #P < 0.01 versus  control 60 min reoxygenation group. §P < 0.05 versus  control 15-min reoxygenation group. GAPDH = glyceraldehyde 3-phosphate dehydrogenase; Prava = pravastatin; reox = reoxygenation.