Fig. 1. Changes of pain behaviors of the hind limb over time in tumor-bearing mice and sham group mice. The number of spontaneous flinches during 2 min (A ) progressively increased as time went on after day 7 in tumor-bearing mice. Paw withdrawal mechanical threshold to von Frey filaments (B ) and paw withdrawal thermal latencyto radiant heat (C ) progressively decreased as time went on after day 7 in tumor-bearing mice. The contralateral limb showed no changes in pain behaviors. Ipsi = ipsilateral hind limb to the surgery; Contra = contralateral hind limb to the surgery. Each group used eight mice. Data were presented as mean ± SD. #P < 0.05 versus day 0. *P < 0.05 versus sham mice.