Fig. 3. Effects of Ro27-3225 treatment on expression of target genes evaluated using real-time polymerase chain reaction in rat livers. Ro27-3225 treatment reduced expression of activating transcription factor 3, early growth response 1, heme oxygenase (decycling) 1, FBJ murine osteosarcoma viral oncogene homolog, and jun oncogene at 1 h; expression of alpha-2-macroglobulin, heat shock 70kD protein 1A, erythropoietin, and interleukin-6 was significantly reduced by treatment at 3 h. Bars denote mean ± SEM (n = 6). **P < 0.01, ***P < 0.001. A2m = alpha-2-macroglobulin; Atf3 = activating transcription factor 3; Egr1 = early growth response 1; Epo = erythropoietin; Fos = FBJ murine osteosarcoma viral oncogene homolog; Hmox1 = heme oxygenase (decycling) 1; Hspa1a = heat shock 70kD protein 1A; Il6 = interleukin-6; Jun = jun oncogene.