Fig. 2. Protein kinase Cα, β, γ, or δ knockout mice show no statistically significant difference in their performance on the hot-plate test (A ), tail-flick test (B ), Hargreaves test (C ), or von Frey Filament test (D ) compared with wild-type littermate control mice (group main effects). Details of each test are provided in Materials and Methods. For all experiments, the experimenter was blind to genotype of the animals. KO = knockout; WT = wild type.