Fig. 3. js127 is a gain of function acy-1 allele. js127 dominantly suppresses the locomotion defect of e246. js127/+ indicates a heterozygous genotype (*P < 0.01 vs. corresponding e246 value) (A ). js127 dominantly increases aldicarb sensitivity. js127/+ is significantly hypersensitive to 0.35 mM aldicarb compared with wild type (P < 0.05) (B ). acy-1(nu329 rf) is a reduction of function allele of acy-1 27and is hypersensitive to isoflurane. The EC50for wild type is 1.05 ± 0.7 compared with 0.58 ± 0.08 for nu329 (P < 0.002) (C ). acy-1 (+) and L244S transformants do not confer isoflurane resistance. jsEx570 [acy-1(+ )]and jsEx571 [acy-1(+ )], wild-type worms transformed with the acy-1 (+) and jsEx575 [acy-1(L244S )] had wild-type sensitivities to isoflurane (*P < 0.01 vs. wild type) (D ). acy-1 (+) and L244S transformants do not suppress the sluggish locomotion of unc-64(e246 ) (*P < 0.01 vs. unc-64(e246 ) (E ). Cyclic adenosine monophosphate (cAMP) levels increased in js127. Endogenous cAMP levels in wild type, js127 , and nu329 adult animals normalized to total protein (*P < 0.05 vs. wild type) (F ).