Fig. 4. Propofol overdose deregulates the expression of Bcl-2 family proteins. RAW264.7 cells (1 × 106cells/well in 6-well culture plates) were treated with propofol (25 μg/ml) or vehicle for the indicated time periods. (A ) Western blot analysis was used to determine the expression of Mcl-1, Bcl-2, Bcl-xL, and active Bax. β-actin was the internal control. The ratios of these proteins to β-actin are shown compared with the normalized vehicle group. Data are representative of three individual experiments. (B ) A quantitative accumulated Western blot data has been shown. Data, obtained from three individual experiments, are means ± SD. *P < 0.05 compared with the control group.