Fig. 5. CaMKIIδ (56 kDa) phosphorylation in single (A ) and combination treatments (B ). CaMKIIδ activity was estimated from the ratio of p-CaMKIIδ and total CaMKIIδ. Actin (42 kDa) was used as loading control. CTL, time-matched aerobic control; IR, ischemia–reperfusion alone; SEVO+IR, periischemic sevoflurane treatment (2 vol.-%); PROP+IR, periischemic propofol treatment (10 μM); REMI+IR, periischemic remifentanil treatment (3 nM); SEVO/PROP+IR, periischemic sevoflurane (2 vol.-%) plus propofol (10 μM) treatment; REMI/PROP+IR, periischemic remifentanil (3 nM) plus propofol (10 μM) treatment; REMI/SEVO+IR, periischemic remifentanil (3 nM) plus sevoflurane (2 vol.-%) treatment. *P < 0.05 compared with IR. #P < 0.05 compared with PROP+IR or SEVO/PROP+IR. §P < 0.05 compared with CTL. Data are mean ± SD.