Fig. 5. MyD88−/−mice had attenuated peritoneal lavage and serum cytokine levels during polymicrobial sepsis. WT and MyD88−/−mice underwent sham or CLP procedures. Twenty-four hours later, peritoneal lavage and blood were collected. Six cytokines, namely IL-6, IL-10, TNFα, KC, MCP-1, and MIP-2, in the peritoneal lavage and sera were measured using a multiplex fluorescent bead-based immunoassay. (A ) Peritoneal lavage cytokines. (B ) Serum cytokines. Each error bar represents mean ± SE. The number of animals in each group: WT-Sham = 4, WT-CLP = 4, MyD88−/−-Sham = 3, MyD88−/−-CLP = 3. CLP = cecum ligation and puncture; IL = interleukin; TNF = tumor necrosis factor; KC = keratinocyte chemoattractant; MCP-1 = monocyte chemoattractant protein-1; MIP-2 = macrophage inflammatory protein-2; MyD88 = myeloid differentiation factor 88; Trif = TIR-domain-containing adaptor protein inducing interferon-β mediated transcription-factor; WT = wild-type. *P < 0.05; **P < 0.01; ***P < 0.001.