Fig. 6. Caveolin-1 (Cav-1) maintains isoflurane-mediated glycolysis after tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Extracellular acidification rate, a measure of glycolysis, and oxygen consumption rate were assessed in HT29-GFP cells. Isoflurane exposure increased extracellular acidification rate basally and after oligomycin but not after TRAIL. No differences were observed in oxygen consumption rate (A ). Extracellular acidification rate and oxygen consumption rate were measured in HT29-Cav-1 cells. Isoflurane exposure increased the extracellular acidification rate basally and after oligomycin. This increased glycolysis was maintained after TRAIL in HT29-Cav-1 cells. No differences were observed in oxygen consumption rate (n = 10 for all groups) (B ). *P < 0.05; **P < 0.01; ***P < 0.001. ECAR = extracellular acidification rate; GFP = green fluorescent protein; Iso = isoflurane; OCR = oxygen consumption rate.