Fig. 1. The effects of brief isoflurane exposure in the human colon cancer cell lines HCT116 and HT29. Cav-1 is up-regulated in HCT116 cells and down-regulated in HT29 cells. Actin was used as a loading control (A ). Cells were exposed to isoflurane (1.2%, 30 min) and enrichment factor, a measure for apoptosis, was evaluated at 0, 6, and 24 h after treatment with isoflurane (B ). Brief isoflurane alone did not show proapoptotic effect at any time point relative to vehicle (five independent experiments were performed). Cav-1 = caveolin-1.