Fig. 5. Reactive oxygen species (ROS) production in submitochondrial particles (SMP) with complex I (NADH) and complex II (succinate) substrates in the presence or absence (Con) of isoflurane (Iso). Representative traces (A ) show the effects of Iso on ROS production using the different substrates (S). Iso induced ROS generation only when NADH was used as substrate, but had no effect when succinate (Succ) was used. (B ) Summary data are mean ± SD, n = 8/group. *P < 0.05 versus Con.