Fig. 3. L-655,708 does not affect minimum alveolar concentration (MAC) in mice. Preinjection of L-655,708 did not alter the MAC value of isoflurane as measured using the tail-clamp assay compared with vehicle-treated controls. Dose-response plots for the immobilizing dose of isoflurane revealed no difference in the EC50values estimated from the fitted curves (mean ± SEM, 1.32 ± 0.048 vs. 1.32 ± 0.048, n = 17 per group) for L-655,708-treated and vehicle-control mice, respectively.