Fig. 4. Production of MIF by the spinal neurons after CCI injury. Immunofluorescent colocalization revealed that MIF coexists with NeuN (G -I ; n = 5), but not with CD11b or GFAP (A -F ; n = 5) in the ipsilateral spinal cord dorsal horn after CCI injury (red color: Texas Red-conjugated antibody; green color: FITC-conjugated antibody; scale bar: 40 μm). MIF = macrophage migration inhibitory factor; CCI = chronic constriction sciatic nerve injury; FITC = fluorescein isothiocyanate; GFAP = glial fibrillary acidic protein; NeuN = neuronal nuclei.