Fig. 6. Isoflurane enhances ROS production in human embryonic stem cell (hESC)-derived cardiomyocytes. Reactive oxygen species (ROS) production in hESC-derived cardiomyocytes was measured by detecting fluorescence intensity of ethidium (Eth). Compared with the time control, isoflurane (Iso) significantly increased Eth fluorescence intensity. In group Iso, three observations in the first two time points of baseline and five observations in the last time point of treatment are missing. *P < 0.05 versus time control and baseline.