Fig. 6.  (A ) Representative immunoblot depicting the effect of propofol (10 μm) alone, propofol in the presence of the protein kinase C epsilon (PKCϵ) inhibitor peptide ϵV1-2 (0.5 μm), PKCϵ activator peptide ψϵRACK (0.5 μm), or Intralipid (amount equivalent to 10 μm propofol) alone on PKCϵ serine 729 phosphorylation (PKCϵpS⁷²⁹) in mouse dorsal root ganglion (DRG) neurons. Total PKCϵ was used as a loading control. (B ) Summarized data for A . Data are expressed as a percentage of the untreated control. *P < 0.05 compared with control. #P < 0.05 compared with propofol. n = DRG cell lysates from five different mice. (C ) Representative immunoblot depicting the effect of propofol (10 μm) alone, propofol in the presence of ϵV1-2 (0.5 μm) or ψϵRACK (0.5 μm) alone on PKCϵ membrane association in mouse DRG neurons. Porin was used a membrane fraction loading control. C = cytosolic fraction; M = membrane fraction. (D ) Summarized data for C . Data are expressed as a percentage change in PKCϵ redistribution from the cytosolic to membrane subfraction. *P < 0.05 compared with control membrane fraction. n = DRG cell lysates from five different mice.