Fig. 1. (A ) Knockdown of subcutaneous NR1 subunit does not affect motor coordination as measured by the rotarod test. No significant difference was noted among the four groups (n = 6 each group). (B ) Knockdown of subcutaneous NR1 subunit 3 days after injection of NR1-1 siRNA increases withdrawal threshold in the complete Freund's adjuvant (CFA)-induced inflamed hind paw (n = 5 each group). S = saline; PEI = polyethyleneimine; MM = mismatched NR1-1 siRNA; Contra = injection of CFA in the paw contralateral to the siRNA-injecting paw; CFA 1 D = measurement of withdrawal threshold 1 day after injection of CFA. *P < 0.05 compared with baseline data. Values are mean ± SD.