Fig. 7. 2-Aminoethoxydiphenyl borate (2-APB) attenuates sevoflurane-induced caspase-3 activation and Aβ accumulation in the brain tissues of neonatal naïve mice. (A ) Anesthesia with 3% sevoflurane for 6 h (lanes 2 and 3) induces caspase-3 cleavage (activation) when compared with the control condition (lane 1). 2-APB (5 mg/kg: lanes 6 to 8; and 10 mg/kg: lanes 4 and 5) attenuates sevoflurane-induced caspase-3 activation. (B ) Quantification of the Western blot shows that sevoflurane anesthesia (black bar ) induces caspase-3 activation compared with the control condition (white bar ). 2-APB (5 mg/kg: gray bar ; and 10 mg/kg: hatched bar ) attenuates sevoflurane-induced caspase-3 activation (black bar ). (C ) Sevoflurane anesthesia (lanes 3 and 4) increases Aβ levels when compared with the control condition (lanes 1 and 2). 2-APB treatment (lanes 5 and 6) attenuates sevoflurane-induced increases of Aβ levels (lanes 3 and 4). (D ) Quantification of the Western blot shows that sevoflurane anesthesia (black bar ) increases Aβ levels compared with the control condition (white bar ). 2-APB treatment (10 mg/kg; hatched bar ) attenuates sevoflurane-induced increase of Aβ (black bar ). We have averaged the results from four independent experiments. Aβ=β-amyloid protein; FL = full length. *P < 0.05 or # P < 0.05; **P < 0.01 or ## P < 0.01.