Fig. 4. Effects of isoflurane or sevoflurane on cell cycle regulatory proteins, β-site amyloid β-precursor protein-cleaving enzyme (BACE), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Proteins from cerebral cortex of neonatal mice were used for Western blotting to compare their changes among different experimental groups. (A , C , E , G ) Representative changes of cell cycle regulatory proteins (CDK4, Cyclin D1), BACE, and GAPDH in the Western blot listed on the left panel . (B , D , F , H ) Statistical analysis and comparison of changes of four proteins listed on the right panel . Each protein was quantified and normalized to the loading control β-actin and compared among control, isoflurane, and sevoflurane groups. Data represents mean ± SE. N = 3 for control, isoflurane, and sevoflurane. con = control, iso = isoflurane, sevo = sevoflurane.