Fig. 3. Isoflurane induced greater apoptosis than equipotent exposure of sevoflurane in the cerebral cortex of neonatal developing brains. (A , C ) Representative changes of cleaved caspase-3 (A ) and poly-(ADP-ribose) polymerase (PARP) (C ) in cerebral cortex by Western Blot using specific antibody targeted to cleaved caspase-3 and PARP, respectively. (B , D ) The quantified cleaved caspase-3 (B ) and PARP (D ) bands were normalized to the loading control β-actin. N = 6 for control, isoflurane, and sevoflurane groups. *P < 0.05 compared with control. **P < 0.01 or ***P < 0.001 compared with control. ## P < 0.01 compared with sevoflurane treatment. con = control, iso = isoflurane, sevo = sevoflurane.