Fig. 1. Visualization of dendritic arbor in layer 5 pyramidal neurons of the medial prefrontal cortex. A , Low-power photomicrograph reveals Lucifer Yellow-injected pyramidal neurons in layer 5 of the medial prefrontal cortex. B , High-power view showing detailed dendritic arbor architecture of a typical layer 5 pyramidal neuron in this cortical region. C , Reconstruction of this same pyramidal neuron using the Neurolucida software (Microbrightfield, Williston, VT). D , Confocal imaging of dendritic shafts allows accurate visualization of dendritic spines. Correction bar: main micrograph, 500 μm; upper insert, 50 μm; lower insert, 3 μm.