Fig. 1. Electroencephalographic (EEG) activity in rat pups during sevoflurane anesthesia. (A ) An illustration of the EEG electrode placement for continuous EEG recordings from bilateral occipital (EEG1) and right frontal (EEG2) regions using an EEG/electromyogram system (Pinnacle Technology, Lawrence, KS). (B ) Examples of EEG recordings in postnatal days 4 and 17 (P4 and P17) rats during start of anesthesia induction with 6% sevoflurane and during anesthesia maintenance with 2.1% sevoflurane. (C ) An example of EEG seizures recorded from a 5-day-old male rat during anesthesia with 2.1% of sevoflurane (see Results for details). REF = reference electrode.