Fig. 3. Nuclear factor (NF)-kB activity in lung tissue. NF-κB activity in lung tissue of ventilated (V) wild type (WT) and Toll/interleukin-1 receptor domain-containing adapter-inducing interferon-β (TRIF) mutant mice. In ventilated TRIF mutant mice (group V-TRIF), nuclear factor-κB activity was significantly lower (  P < 0.001) compared with ventilated WT mice (group V-WT). n = 8 for all groups. Data are expressed as optical densities from electrophoretic mobility shift assay analysis. +=  P < 0.05 compared with ventilated WT mice (V-WT).