Fig. 6. Renal dimethylarginine dimethylaminohydrolase (DDAH) activity. Homogenates were centrifuged at 5,000  g for 60 min, and supernatants were used to determine DDAH enzyme activity, which was assayed by determining l-citrulline formation in a 96-well microtiter plate. Values are the mean ± SD of four experiments in duplicate (*  P < 0.05  vs. sham-operated animal).