Fig. 4.  (A ) Representative sections of nuclear DNA fragmentation assays performed by terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) staining in the penumbral cortex in sham, control (CON) and 30 μg/kg orexin-A (OXA) treatment groups (n = 6). (B ) Quantitative analysis of the number of TUNEL-positive neurons in the penumbral cortex. Data are presented as mean ± SD. Scale bar, 10 μm. #P < 0.05 versus  sham. *P < 0.05 versus  control.