Fig. 2.  Neurologic scores and infarct volume after 120 min of transient middle cerebral artery occlusion in sham, control (CON), and orexin-A treatment groups. Orexin-A was administered at the onset of reperfusion by intravenous injection. Neurologic scores are presented as median (range); data for infarct volume are expressed as mean ± SD. Neurologic scores were evaluated at 24 h (A ) and at 72 h (B ) after reperfusion using the Garcia scoring system. (C ) Representative 2,3,5-triphenyltetrazolium chloride staining of the cerebral infarct in the rat brain at 72 h after reperfusion. (D ) Statistical analysis of the percentage of infarct volume was determined for each study group (n = 8). OXA-3 = 3 μg/kg orexin-A; OXA-10 = 10 μg/kg orexin-A; OXA-30 = 30 μg/kg orexin-A; OXA-100 = 100 μg/kg orexin-A. *P < 0.05 versus  control. **P < 0.01 versus  control.