Fig. 5. Effect of ventilation and hydrogen sulfide (H2S) treatment on stress protein expression. As controls, mice were subjected to spontaneously breathe air or 80 ppm H2S or were ventilated (vent) with 12 ml/kg for 6 h with either synthetic air or synthetic air + 80 ppm H2S. Lung samples were taken from the upper right lobe for Western blot analysis. (A ) Representative Western blots for heme oxygenase-1 (HO-1; upper ), heat shock protein 70 (HSP70; middle ), and glyceraldehyde-3-phosphate-dehydrogenase (GAPDH; lower ). (B , C ) Densitometric analysis of all samples were normalized to GAPDH and expressed as fold induction for HO-1 (B ) and HSP70 (C ). Graphs represent means ± SEM, n = 5/group. Analysis of variance (Student-Newman-Keuls post hoc test), *P < 0.05 versus control; # P < 0.05 versus H2S control; §P < 0.05 versus H2S-ventilated group.