Fig. 3. Influence by prolonged treatment with Chembridge-5861528 (CHEM, a TRPA1 channel antagonist) on pain-related behavior in diabetic animals. ( A ) Paw pressure threshold of diabetic animals treated for a week either with CHEM or vehicle (Veh). ( B ) Body weight in diabetic animals during prolonged treatment with CHEM or vehicle. D3-D10 indicate numbers of days from the induction of diabetes mellitus (DM), and pre refers to time point before its induction. Prolonged treatment with CHEM (30 mg/kg twice daily) or vehicle started on D3 and ended on D10. Paw pressure threshold was determined in each day 6 h after administration of the morning treatment with CHEM or vehicle; i.e. , paw pressure threshold was determined at a time point when the acute treatment effect by CHEM was over. Error bars represent SEM (n = 9 in each group). ** P < 0.01, *** P < 0.005 ( t test with a Bonferroni correction for multiple comparisons; reference: the corresponding value in the Veh-group).