Fig. 4. Effect of Propofol and phorbol myristic acetate (PMA) on intrinsic fluorescence of protein kinase Cϵ (PKCϵ) C1B subdomain. ( A ) Left = Representative emission spectra depicting the effect of 10 successive additions of 0.1 μm propofol (0.1–1.0 μm) on the intrinsic fluorescence of the PKCϵ C1B subdomain (1 μm). Right Summarized data depicting effect of propofol (0.1–1.0 μm) or intralipid (1 μm) on intrinsic fluorescence of PKCϵ C1B subdomain. *P < 0.05 versus PKCϵ C1B subdomain alone (untreated control). n = 6. ( B ) Left = Representative emission spectra depicting the effect of 10 successive additions of 0.1 μm PMA (0.1–1.0 μm) on the intrinsic fluorescence of the PKCϵ C1B subdomain (1 μm). Right = Summarized data depicting the effect of successive additions of PMA on intrinsic fluorescence of PKCϵ C1B subdomain. *P < 0.05 versus PKCϵ C1B subdomain alone (untreated control) n = 6.