Fig. 1. Effect of propofol on autophosphorylation of recombinant protein kinase Cϵ (PKCϵ) at Ser⁷²⁹before and after treatment with λ phosphatase. (  A ) Western blot analysis of recombinant PKCϵ depicting the effect of intralipid (intralipid; 10 μm), phorbol myristic acetate (PMA; 0.1 μm), or propofol (Prop; 1 μm) on immunodetectable PKCϵpS⁷²⁹(pPKCϵ) and total PKCϵ before treatment with λ phosphatase. (  B ) Same as  A , except after treatment with λ phosphatase. (  C and  D ) Summarized data for  A and  B , respectively. Ctrl = control value in absence of intralipid, PMA, or Prop. *P < 0.05  versus untreated Ctrl. #  P < 0.05  versus phosphatase-treated Ctrl n = 6.