Fig. 5. Currents induced by propofol (IPRO) from isolated hypothalamic neurons. ( A1 ) Representative current traces induced by various concentrations of propofol. ( A2 ), Peak amplitude (mean ± SEM of 4 to 12 cells) of the IPROagainst the concentrations of propofol. The solid line is least square fit of the Michaelis-Menten equation described in fig. 3 to the experimental data. The EC50and Hill coefficient values are 26.4 ± 3.4 μm and 1.8 ± 0.4, respectively. ( B ) Representative traces of propofol-induced current in the presence of strychnine (1 μm) ( B1 ), bicuculline (10 μm), or both ( B2 ). ( B3 ) Mean ± SEM for strychnine or bicuculline inhibition of propofol-induced current.