Fig. 4. ( A ) Representative immunoblots of homogenates of left ventricles from the Sham, Control, low-dose propofol (Prop-LD), high-dose propofol (Prop-HD), propofol with atropine (Prop-Atr), and sevoflurane (Sevo) groups, probed with polyclonal Cx43 antibody. Arrows indicate the position of phosphorylated isoform of Cx43 (P-Cx43; 43 kDa) and nonphosphorylated isoform of Cx43 (NP-Cx43; 41kDa) bands, respectively. ( B ) Optical density analysis of P-Cx43:actin (□) and NP-Cx-43: actin (▪) ratios in the Control, Prop-LD, Prop-HD, Prop-Atr, and Sevo groups, respectively. Data are expressed as mean ± SD from 5 rats in each group. * P < 0.05. † P < 0.01 versus Cx43 in the Control group.