Fig. 5. Effect of cardiopulmonary bypass (CPB) on GRK6 expression in mononuclear cell membranes (n = 12). (A ) GRK6 immunoreactivity in mononuclear cell membranes from patients 3 and 1. The immunoblotting was carried out as described in the methods section. GRK6 lane, 2 μg of recombinant GRK6 in Sf9 membranes; PRE lanes, 30 μg mononuclear cell membrane proteins from pre-CPB sample; POST lanes, 30 μg mononuclear cell membrane protein from post-CPB samples; POD1 lanes, 30 μg mononuclear cell membrane protein from POD1 samples. (B ) The GRK6 protein expression in POST and POD1 samples from individual patients. Patient numbers are shown to the right. PRE = before surgery; POST = 2 h after completion of CPB; POD1 = 24 h after completion of CPB.