Fig. 3. (A ) Platelet-activating factor (PAF)-primed–N-formylmethionine-leucyl-phenylalanine (fMLP)-activated O2− production by hPMNs (black bar) is inhibited after 60-min pretreatment with bisindolylmaleimide (BIM; gray bar) or chelerythrine (Ct; gray bar) to a level not significantly different from that obtained with fMLP activation alone (white bar). (B ) Concentration–response relation for superoxide anion production induced by various concentrations of phorbol myristate acetate (PMA; 1–200 nm) measured 40 min after adding PMA to human polymorphonuclear neutrophils (hPMNs). (C ) Time course of superoxide anion production induced by either fMLP activation and previous incubation with PAF for 5 min, PMA alone, or PMA and 5-min preincubation with PAF. See text for details. n.s. = not significant.
