Fig. 1. Dissociation of FK506 binding protein from sarcoplasmic reticulum terminal cisternae vesicles (SRVs). One milligram SRVs per milliliter was incubated at 37°C for 40 min without or with 25 μm FK506. After incubation, SRVs were centrifuged for 30 min at 50,000 g in a Beckman 50.4 Ti rotor (Palo Alto, CA) at 4°C, and the supernatant was collected. The figure shows the SDS-PAGE and western blot analysis for FK506 binding protein 12 in the collected supernatant of control (lanes 1 and 2) and FK506-treated (lanes 3 and 4) SRVs.