![Figure 7. Quenching of human serum albumin (hSA) fluorescence by F6. Fluorescence intensity obtained after equilibrating 5 [micro sign]M hSA or 5 [micro sign]M free L-tryptophan with various concentrations of F6. The solid line is a fit of the hSA fluorescence data to the binding isotherm given in Equation 7. The apparent Kdfor F6 = 160 +/- 11 [micro sign]M, and the Qmax= 1.05 +/- 0.038. The dotted line is a linear least-squares fit of the L-trytophan fluorescence data.](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/88/6/10.1097_00000542-199806000-00018/4/18ff7.png?Expires=1716449121&Signature=TVPlmNapmZkX-g9iOPOMrhOviGWgA~C2WAFy2vGBvz~I0SAUmHlv9q-tdxrPANzDcsAw3SDUPu7q5vZL2XnuGwM9jE9f2QcEw75WprwYESPxNGZYUdPMbXgnP~4bUpe0BGgB29y~HNxeofNLj4jKyaz8jhT0baIfvR1zmPIxvsu048QoLqnYbCCp0D0WaToHyrb3KTiinh9t5hFfUFE7C1SzNnholdrnM6hu2wdVTWGrVv3kn4X13DtHC6nzn9OKKb5iUva5bd9FLXrNjeT9RR94RVXtn~zj-DG128AQcYQJ7sPKqx8B0qxmDBxAb69D9tJuwSuDEn4lB6F2Guq6WA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 7. Quenching of human serum albumin (hSA) fluorescence by F6. Fluorescence intensity obtained after equilibrating 5 [micro sign]M hSA or 5 [micro sign]M free L-tryptophan with various concentrations of F6. The solid line is a fit of the hSA fluorescence data to the binding isotherm given in Equation 7. The apparent Kdfor F6 = 160 +/- 11 [micro sign]M, and the Qmax= 1.05 +/- 0.038. The dotted line is a linear least-squares fit of the L-trytophan fluorescence data.
