Figure 1. Time courses (A) and dose-dependency (B) for the effect of intrathecal injection of strychnine and bicuculline on allodynia. Studies on allodynia were conducted as described in materials and methods. Mice were injected with 0.25 micro gram strychnine (open circle) and 1.25 micro gram bicuculline (closed circle). Each column in A represents the percentage of the maximum possible cumulative score of six to eight mice evaluated every 5 min (mean+/-SE). The values (mean+/- SE, n = 6-8) of allodynia shown in B are expressed as a percentage of the maximum possible score over the 50-min observation period following different doses (12.5 ng-2.5 micro gram).