Figure 5. Autoradiograph of a Western blot showing the effect of anesthesia and surgery on hepatic steady-state heat-shock protein-72 (hsp72) levels. Total cellular proteins were isolated from serial pig liver biopsy samples that had been obtained at baseline (time 0), and at 1, 2, 3, 4, and 6 h after baseline, and were probed with a monoclonal antibody specific for the inducible form of heat-shock protein-70 (heat-shock protein-72; hsp72). Samples of clarified liver homogenates were subjected to sodium dodecyl sulfate-polyacrylamide (10%) gel electrophoresis (100 micro gram protein/lane), transferred to nitro-cellulose membranes, probed with the monoclonal anti-hsp72 antibody C92F3A-5, tagged with a125Iodine-labeled goat-anti-mouse antibody, and autoradiographed. The protein extracts used for this Western blot, the RNA processed for the Northern blot shown in Figure 3, and the hepatic nuclei used for the runoff analysis shown in Figure 1were all obtained from the same animal.